李晓娜 刘成星 王晓君

中文摘要:
      作为检测生物软材料和活细胞黏弹性特性的一种新方法，粒子跟踪微流变学主要应用于细胞运动、胚胎发育、核纤层蛋白病等生物力学研究中，与以往传统的细胞力学检测手段相比具有很多优势。其利用视频显微镜检测包埋于介质中探针的布朗热运动，将探针运动轨迹转换平均平方位移，实时获取介质的时间依赖性黏弹性模量和蠕变柔量等参数。本文主要对粒子跟踪微流变学技术的基本原理及其在生物力学领域的应用进行了综述。
英文摘要:
      As a new technique of determining the viscoelasticity of soft biomaterials and cell cytoplasm in living cells, particle tracking microrheology (PTM) is mainly applied in the biomechanical research field, such as cell movement, embryo development, laminopathies. PTM has many advantages over the conventional detection methods in cell mechanics. Using this technique, the Brownian motion of probe particles embedded in the medium could be measured by the video-microscopy, and the movement trajectories of the probe could be mathematically transformed into the mean squared displacements (MSDs) thus to extract the parameters such as the frequency-dependent viscoelastic modulus or the creep compliance from the timedependent MSDs of the probes. The basic principles of PTM technique and its application in biomechanics will be reviewed in this paper.